Specialty Guide · Path-iQ Global Pathology Review

Paediatric Pathology
The Complete Guide 2026

Paediatric pathology is the subspecialty concerned with diseases of infants, children, and adolescents — including tumours, developmental anomalies, perinatal pathology, inherited metabolic disorders, and sudden unexpected death in childhood. It requires mastery of normal developmental anatomy at every age from foetus to adolescence, making it one of the most knowledge-intensive and intellectually demanding subspecialties in anatomic pathology.

Updated 30 July 2026 · Path-iQ Editorial · About Path-iQ →

Paediatric vs Adult Tumours — Key Differences

Childhood cancers differ fundamentally from adult cancers in their biology, histology, genetic drivers, and prognosis. Whereas adult cancers arise from accumulated somatic mutations over decades, most childhood tumours arise from aberrant developmental programmes — oncofetal genes, disrupted differentiation, or germline predisposition syndromes — and frequently carry single or few high-impact genetic events rather than the complex mutational landscapes of adult carcinomas.

FeaturePaediatric CancersAdult Cancers
Primary tumour typeLeukaemia (most common), brain tumours, lymphoma, neuroblastoma, Wilms, soft tissue sarcoma, retinoblastoma, Ewing sarcomaCarcinoma (breast, lung, prostate, colorectal) dominates; carcinoma rare in childhood
Mutational burdenVery low (<1 mut/Mb in most paediatric tumours); often single driver eventHigh (10–1000 mut/Mb in carcinoma); multiple sequential mutations over years
CarcinomaExtremely rare; adrenocortical carcinoma, hepatocellular carcinoma (HBV-related), NPC in adolescentsCommonest malignancy type worldwide
Embryonal tumoursFrequent — Wilms tumour, neuroblastoma, hepatoblastoma, medulloblastoma, retinoblastoma all embryonal in originAbsent (embryonal tumours resolve or transform by adulthood)
Overall prognosisMuch better — 5-year survival >85% in high-income countries for all childhood cancers combinedHighly variable; many adult cancers have 5-year survival <50%
Treatment responseOften exquisitely chemosensitive; high complete response ratesChemoresistance common in carcinomas; targeted therapy required
Hereditary predispositionRecognised in ~10% of childhood cancers (RB1, TP53 Li-Fraumeni, WT1, DICER1, SMARCA4, BRCA2, Beckwith-Wiedemann)BRCA1/2, Lynch syndrome ~5–10% of adult cancers; majority sporadic

Major Paediatric Tumour Entities

Wilms Tumour (Nephroblastoma)

Wilms tumour is the most common primary renal tumour of childhood (peak age 3–4 years; 95% of paediatric renal tumours). The classic triphasic histology consists of blastemal, stromal, and epithelial components in varying proportions. Histological risk stratification drives treatment intensity:

Neuroblastoma

Neuroblastoma is the most common extracranial solid tumour of childhood, arising from neural crest-derived sympathetic precursors (adrenal medulla ~50%; paraspinal sympathetic chain; neck, chest, pelvis). The International Neuroblastoma Pathology Classification (INPC / Shimada system) stratifies tumours into favourable and unfavourable histology based on degree of Schwannian stromal development, differentiation, and mitosis-karyorrhexis index (MKI).

INPC CategoryHistologyAge / MKI CriteriaPrognosis
Neuroblastoma, FHPoorly differentiated or differentiating; <50% Schwannian stromaAge <1.5yr + low/intermediate MKI; OR age <5yr + low MKIFavourable; excellent with risk-adapted therapy
Neuroblastoma, UFHUndifferentiated OR high MKI for ageUndifferentiated at any age; OR age >1.5yr + intermediate/high MKIUnfavourable; high-risk treatment required
Ganglioneuroblastoma, intermixed>50% Schwannian stroma; nests of neuroblasts within stromaAny ageFavourable
Ganglioneuroblastoma, nodularComposite: ganglioneuroma / stroma-rich + neuroblastoma nodule(s)Nodule histology determines risk (FH vs UFH)Variable; determined by neuroblastoma nodule
Ganglioneuroma>50% mature Schwannian stroma; fully mature ganglion cells; no neuroblastsAny age; represents maturation of neuroblastomaBenign; surgical excision curative

MYCN amplification (>10 copies / cell by FISH) is the single most important adverse prognostic molecular marker in neuroblastoma, present in ~25% of cases and associated with aggressive biology. Additional molecular risk factors: ALK mutation/amplification; segmental chromosomal aberrations (1p del, 11q del, 17q gain); DNA ploidy (hyperdiploid = favourable in infants).

Hepatoblastoma

Hepatoblastoma is the most common primary liver tumour in children (peak age 1–2 years; rare after age 5). PRETEXT (pre-treatment extent of disease) staging by imaging guides surgical and chemotherapy planning. Histological subtypes per CHIC criteria: epithelial (pure fetal, embryonal, macrotrabecular, small cell undifferentiated) and mixed epithelial-mesenchymal. Pure fetal histology with low mitotic rate (<2/10 HPF) is associated with the best prognosis; small cell undifferentiated (SCUD) with INI1 loss (SMARCB1 inactivation) is highest risk.

Retinoblastoma

Retinoblastoma arises from immature retinal precursors (retinoblasts) in children almost exclusively under age 5. Bilateral retinoblastoma (40% of cases) is virtually always hereditary, caused by germline RB1 mutation. Unilateral cases may be hereditary or sporadic. Histological high-risk features that predict metastasis and mandate adjuvant chemotherapy: massive choroidal invasion (>3 mm); optic nerve invasion posterior to the lamina cribrosa; scleral or anterior segment invasion; surgical margin involvement.

Common Paediatric Brain Tumours

TumourPeak AgeLocationKey Molecular FeaturePrognosis
Pilocytic astrocytoma (WHO grade 1)5–15 yrCerebellum (most common); optic pathway; hypothalamusKIAA1549-BRAF fusion (~70% cerebellar); BRAF V600E (optic pathway); RAF1 fusionsExcellent; surgical cure in most cerebellar cases; 10-yr OS >95%
Medulloblastoma (WHO grade 4)5–9 yr (childhood); bimodal with adultsPosterior fossa (cerebellum / fourth ventricle)WNT (best; CTNNB1); SHH (intermediate; TP53 status splits risk); Group 3 (worst; MYC amp); Group 45-yr OS: WNT >90%; SHH-TP53 wildtype 75%; Group 3 ~50%; Group 4 ~75%
Ependymoma (WHO grade 2–3)Bimodal: <5yr (infratentorial) and adultsPosterior fossa (ZFTA-RELA in children: supratentorial); spinal in adultsZFTA-RELA fusion (supratentorial, children, grade 3); YAP1 fusion (supratentorial, infants, better prognosis); PFA/PFB methylation groups (posterior fossa)PFA (posterior fossa A) = worst; 5-yr OS ~65%; PFB = best
Diffuse intrinsic pontine glioma (DIPG) / DMG5–9 yrPons; thalamus; spineH3 K27M mutation (H3.3 or H3.1); now classified as diffuse midline glioma, H3 K27-alteredWorst CNS tumour in children; median OS 9–15 months; ONC201 shows early promise
Atypical teratoid/rhabdoid tumour (AT/RT)<3 yrPosterior fossa and supratentorialSMARCB1 (INI1) or SMARCA4 loss — diagnostic; three methylation subgroups (TYR, SHH, MYC)Historically dismal (<20% 5-yr OS); intensive multimodal therapy improving outcomes in older children

Perinatal Pathology — Placental Examination

Placental pathological examination is indicated after all stillbirths, severe neonatal morbidity, IUGR, preterm birth <34 weeks, and preeclampsia with severe features. The Amsterdam Placental Workshop Group Consensus Statement (2016) provides standardised terminology for placental lesions classified by timing (acute vs chronic) and aetiology (maternal vascular malperfusion, foetal vascular malperfusion, inflammation, and other).

Leading Paediatric Pathologists — Global 2026

Megan Lim
Paediatric Haematopathology
University of Pennsylvania / Children's Hospital of Philadelphia

Authority on paediatric lymphoma, leukaemia immunophenotyping, and the WHO classification of haematolymphoid tumours as applied to childhood malignancies. Expert in EBV-associated lymphoproliferative disorders in immunocompromised children.

Elizabeth Fuentes-Alabi
Paediatric Oncology Pathology — Low-Income Settings
National Children's Hospital, El Salvador

Pioneer in improving paediatric cancer diagnosis in Central America, focusing on the pathological classification of childhood leukaemia, Wilms tumour, and retinoblastoma in resource-limited settings. Recipient of SIOP global health awards.

Poul Holm Møller
Perinatal & Developmental Pathology
Odense University Hospital, Denmark

Leading Scandinavian perinatal pathologist specialising in placental pathology, Amsterdam criteria classification, and the pathological investigation of stillbirth in Danish perinatal audit programmes.

Bharat Rekhi
Paediatric Soft Tissue & Bone Tumours
Tata Memorial Centre, Mumbai

Expert in paediatric and young adult sarcoma pathology — Ewing sarcoma, rhabdomyosarcoma, osteosarcoma — with experience in the largest paediatric sarcoma volume in South Asia. Contributor to WHO Classification of Soft Tissue Tumours 5th edition.

Cheryl Coffin
Paediatric Soft Tissue Pathology
Vanderbilt University Medical Center

Authority on paediatric soft tissue tumours, rhabdomyosarcoma pathological classification (embryonal vs alveolar vs pleomorphic), FISH testing for PAX-FOXO1 fusion, and the Children's Oncology Group (COG) sarcoma pathology committee.

Ivo Leuschner
Paediatric Tumour Pathology — Europe
Universitätsklinikum Schleswig-Holstein, Kiel

European authority on paediatric renal and hepatic tumours; contributor to SIOP nephroblastoma working group histological classification and the German Society of Paediatric Oncology pathology committee.

Frequently Asked Questions

How is paediatric pathology different from general surgical pathology?
Paediatric pathology requires knowledge of age-specific normal anatomy at every developmental stage — what is normal in a 28-week premature infant is different from a 2-year-old or a 14-year-old. Many tumours seen in children (neuroblastoma, Wilms tumour, retinoblastoma, hepatoblastoma, medulloblastoma) are essentially never seen in adults, while the carcinomas that dominate adult surgical pathology practice are extremely rare in childhood. Additionally, the inherited and developmental context of paediatric disease requires integration with genetics, dysmorphology, and prenatal history in ways not typical in adult surgical pathology.
What is the SIOP approach to Wilms tumour vs the COG approach?
The International Society of Paediatric Oncology (SIOP) recommends pre-operative chemotherapy before nephrectomy, so the pathologist receives a post-treatment specimen that may show regression, differentiation, or residual anaplasia — making histological risk stratification dependent on treatment response. The Children's Oncology Group (COG) approach favours upfront surgery (primary nephrectomy) before chemotherapy, so the pathologist receives untreated tumour with classic triphasic histology. The two approaches use different histological risk classifications (SIOP includes a "diffuse blastemal" high-risk group that only makes sense post-chemotherapy) and are not directly interchangeable.
When should MYCN testing be performed in neuroblastoma?
MYCN FISH testing should be performed on all newly diagnosed neuroblastoma specimens. MYCN amplification (defined as >10 copies per cell or >4× the reference signal by FISH) is present in ~25% of neuroblastomas and is the single strongest indicator of high-risk biology regardless of stage. Even localised, low-stage tumours with MYCN amplification are treated as high-risk per International Neuroblastoma Risk Group (INRG) criteria. Testing must be performed on adequate tumour tissue (not necrotic or post-chemotherapy residuum) and FISH is the gold standard — PCR-based methods may underestimate amplification.

Related Specialty Guides